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产品介绍
品名	Rat recombinant calbindin D-9k (recombinant) produced in Escherichia Coli
货号	rCB9
规格	10 µg、60 µg
纯化	Ni-chelate chrom
宿主动物	Recombinant rat
抗体形式	lyophil
重组	10 µg protein reconstituted with 25 µl PBS (0.4 µg/µl)  60 µg protein reconstituted with 150 µl PBS
产品描述	E. coli containing a His6-fusion protein were disrupted in a buffer containing 6M GuHCl/0.1 M NaH2PO4. After a low speed centrifugation to remove cell debris, the supernatant was applied to the charged NTA resin column, equilibrated in disruption buffer, before the column being washed with the same buffer. Afterwards, the column was washed and the HIS6- fusion proteins eluted by decreasing the pH of the eluent (8 M Urea /0.1 M Na2HPO4) in a stepwise manner (pH 6.3 / 5.9 / 4 / 2). The protein was dialysed against phosphate buffered saline, 0.1 M, pH 7.3 (PBS)
储存	Freeze in small aliquots (e.g. 1 or 10 µg) and keep at -80°C. Avoid repeated freezing and thawing