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产品介绍
品名	Monoclonal anti-Na+ -Ca2+-exchanger
货号	R3F1
规格	200ul
克隆性	monoclonal
物种反应	human, mouse, rat, sheep
应用	western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, western blot knockout validation
宿主动物	Mouse monoclonal, IgG1
抗体形式	Conc. Supernat lyophil.
产品形式	Lyophilized, concentrated supernatant.
复溶	with 200 µl of bidistilled water.
产品描述	The monoclonal antibody against the canine Na+ -Ca2+-exchanger, recognizes two neighboring but non-overlapping sequences of the hydrophilic region of the exchanger, connecting the putative transmembrane segments 5 and 6. The antibody was evalsuated for specificity and potency by immunoblotting, and immunoprecipitation (see reference). It recognizes virtually only NCX1 in monkey, horse, dog, rat, bovine and mouse tissue. At higher concentrations it reacts faintly with NCX2, whereas NCX3 is not recognized. Both splice variants with exon A (heart, nervous system) and exon B (kidney, glia) are recognized.
稀释	Immunohistochemistry: 1: 500;  1: 1’000 Immunoblots and immunoprecipitation: 1: 500; 1: 1'000 (use milk for blocking). A strong 70 kDa band is found in addition to the 116 kDa of the integral exchanger. Often the HMW band is a double band 116/ 112 kDa band.  The titer was determined using the indirect-peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.
储存	After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
参考文献	Porzig H., Li Z., Nicoll D.A., Philipson K.D. (1993) Mapping the cardiac sodium-calcium exchanger with monoclonal antibodies. Am. J. Physiol. 265:C78 -C756, 1993