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产品介绍
品名	Mouse anti-calretinin (monoclonal)
货号	6B3
规格	200ul
克隆性	monoclonal
共轭	nonconjugated
反应性	Erpetoichthys calabaricus, slender lungfish, Polypterus senegalus, Mongolian jird, human, mouse, rat, zebrafish , Caenorhabditis elegans, rhesus macaque
应用	western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
宿主动物	Mouse monoclonal
抗体形式	Conc. supernat. lyophil.
产品形式	Lyophilized concentrated supernatant (no preservatives).
复溶	each vial with 200 µl of bidistilled water
产品描述	The antibody against calretinin was produced in mice by immunization with recombinant human calretinin-22k (1). Calretinin 22k is an alternative splice product of the calretinin gene and identical with calretinin up to Arg178. After fusion, hybridoma cells were screened with human recombinant calretinin as target and the clone 6B3 was selected. The antibody 6B3 recognizes an epitope within the first 4 EF-hands domains common to both calretinin and calretinin-22k (1). It was evalsuated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffin-sections of 4% paraformaldehyde (or 10% buffered formalin) fixed brains b) by immunoenzymatic labelling of immunoblots c) by immunohistochemistry on tissue of calretinin knock-out mice.
稀释	Immunohistochemistry: 1:2'000 - 1:5'000. On paraformaldehyde (4%) or formalin-fixed tissue. Cryostate or paraffin-sections.  Immunoblots: 1:1'000 - 1:2'000.  For immunohistochemistry and immunoblots the titer was determined by using the avidinbiotin method. We recommend that the optimal dilutions be determined by titration experiments.
储存	After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
参考文献	1. Zimmermann, L. and Schwaller B (2002) Monoclonal antibodies recognizes epitopes of calretinin. Cell Calcium 31: 13-25.  2. Rogers J.H. (1987) Calretinin: a gene for a novel calcium-binding protein expressed principally in neurons. J. Cell Biol. 105:1343-1353.  3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137-147.  4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046.  5. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262.