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产品介绍
品名	Monoclonal anti Calbindin D-28k
货号	300
规格	200ul / 500ul  / 1ml
克隆性	monoclonal
共轭	nonconjugated
反应性	thirteen-lined ground squirrel, Spanish newt, slender lungfish, reedfish , Shaw's jird, gray bichir , African green monkey, human, mouse, rat, chicken, pigs , Xenopus laevis, rhesus macaque
应用	western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
宿主动物	Mouse monoclonal, IgG1
抗体形式	Conc. supernat. lyophil.
产品形式	Lyophilized concentrated supernatant (no preservatives).
复溶	with 200 µl / 500 µl or 1 ml bidistilled water
产品描述	Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evalsuated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay.  The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C
稀释	Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method.  We recommend that the optimal dilutions be determined by titration experiments.
储存	For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
参考文献	1. Celio M.R. et al., Cell Calcium 11:599-602, 1990  2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press  3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86.  4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493