DiscoverX CHK2 (I157T), active 14-741M 14-741M|产品详情|进口橙子视频旧款采购网

您好，欢迎来到橙子视频app安卓下载生物网站！请登录注册

我的订单 | 联系橙子视频app安卓下载 | 在线客服QQ：3012681483 | 客服电话：400-968-7988 | 工作时间：08:30-17:30

主营产品： ELISA91橙子视频、抗体、补体血清、兔补体、干扰素、小鼠测温仪、进口橙子视频旧款、Quidel代理、Swant代理、PBL Assay Science代理

网站首页产品中心代理品牌新闻资讯资源中心关于橙子视频app安卓下载

所在位置：首页 > 产品中心 > discoverx

订购信息

上海橙子视频app安卓下载生物科技公司

Tel:400-968-7988 021-33779008

DiscoverX CHK2 (I157T), active 14-741M

品牌：Eurofins DiscoverX

货号：14-741M

规格：14-741M

货期：DiscoverX

应用：

咨询客服

立即购买加入购物车

品牌：DiscoverX 品名：CHK2 (I157T), active 货号：14-741M 规格：250 µg

商品详情参考文献相关资料

品牌：DiscoverX
品名：CHK2 (I157T), active
货号：14-741M
规格：250 µg

简要说明：

CHK2 (I157T), active

N-terminal GST and C-terminal 6His-tagged,.recombinant human CHK2, amino acids 5-end, containing the I157T mutation. Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.

产品介绍：

Target

CHK2

Expression System

Expressed in E. coli

Application

Kinase Assay

Molecular Weight

89.6 kDa

Accession Number

NM_007194

Species

Human

Tag

GST, 6His

Purification Method

Glutathione agarose affinity chromatography; Ni2+/NTA agarose

Content

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Storage

1 year at -70°C

Bulk Sizes

Inquire

热销产品