Catalogue number
PRO-956
Synonyms
ADP-ribosylation factor-like 15, ADP-ribosylation factor related protein 2, ARFRP2, ARF-related protein 2, FLJ20051.
Introduction
ARL15 is a member of the ARF family which are essential in eukaryotic vesicular trafficking pathways and have a vital part in the activation of phospholipase D. ARL15 variants influence levels of Acrp30, an adipocyte-derived protein that is extremely genetic and inversely related to the prospect of type 2 diabetes mellitus and coronary heart disease.
Description
ARL15 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 224 amino acids (1-204) and having a molecular mass of 25.0 kDa.
ARL15 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The ARL15 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Safety Data Sheet
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MSDLRITEAF LYMDYLCFRA LCCKGPPPAR PEYDLVCIGL TGSGKTSLLS KLCSESPDNV VSTTGFSIKA VPFQNAILNV KELGGADNIR KYWSRYYQGS QGVIFVLDSA SSEDDLEAAR NELHSALQHP QLCTLPFLIL ANHQDKPAAR SVQEIKKYFE LEPLARGKRW ILQPCSLDDM DALKDSFSQL INLLEEKDHE AVRM
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
