Catalogue number
PRO-2209
Synonyms
Annexin A5, ENX2, Placental Anticoagulant Protein 4, Placental
Anticoagulant Protein I, Vascular Anticoagulant-Alpha, Thromboplastin Inhibitor, Calphobindin
I, Anchorin CII, Endonexin II, Lipocortin V,
Annexin V, Annexin-5, VAC-Alpha, RPRGL3, CBP-I, PAP-I, ANX5, PP4, Epididymis
Secretory Protein Li 7, HEL-S-7.
Introduction
ANXA5 is a member of the annexin family of calcium-dependent phospholipid binding proteins which are involved in membrane-related activity along exocytotic and endocytotic pathways. ANXA5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel properties and takes part in cellular signal transduction, inflammation, growth and differentiation. ANXA5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. ANXA5 regulates coagulability in the blood stream by binding to phosphatidylserine and sulfatide. ANXA5 protects sinsuoidal endothelial cells from ischemia reperfusion damage. ANXA5 is necessary for normal CFTR chloride channel activity.
Description
ANXA5 Mouse Recombinant produced in E. coli is a single,
non-glycosylated polypeptide chain containing 342 amino acids (1-319 a.a) and
having a molecular mass of 38.1kDa.
ANXA5 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
ANXA5 protein solution (1mg/ml) containing Phosphate Buffered Saline, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSP MGSMATRGTV TDFPGFDGRA DAEVLRKAMK GLGTDEDSIL NLLTSRSNAQ RQEIAQEFKT LFGRDLVDDL KSELTGKFEK LIVAMMKPSR LYDAYELKHA LKGAGTDEKV LTEIIASRTP EELSAIKQVY EEEYGSNLED DVVGDTSGYY QRMLVVLLQA NRDPDTAIDD AQVELDAQAL FQAGELKWGT DEEKFITIFG TRSVSHLRRV FDKYMTISGF QIEETIDRET SGNLEQLLLA VVKSIRSIPA YLAETLYYAM KGAGTDDHTL IRVVVSRSEI DLFNIRKEFR KNFATSLYSM IKGDTSGDYK KALLLLCGGE DD.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
