Catalogue number
PRO-2244
Synonyms
Cadherin-5,
Vascular endothelial cadherin, VE-cadherin, CD144, Cdh5.
Introduction
Cadherin-5, also known as CDH5 is a classical cadherin which belongs to
the cadherin superfamily. The CDH5 is located in a six-cadherin
cluster in a region on the long arm of chromosome 16 which is involved in loss
of heterozygosity events in breast as well as prostate cancer.
CDH5 is a calcium-dependent cell-cell adhesion glycoprotein
composed of five extracellular cadherin repeats, a transmembrane region and a
highly conserved cytoplasmic tail. Furthermore, CDH5 functions
as a classic cadherin by passing on to cells the ability to adhere in a homophilic manner, CDH5
plays a significant role in endothelial cell biology through control of
the cohesion as well as organization of the intercellular junctions.
Description
CDH5 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 583 amino acids (25-599a.a.) and having a molecular mass of 66.2kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
CDH5 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Physical Appearance
Sterile
filtered colorless solution.
Formulation
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CDH5 protein solution (0.5mg/ml)
contains phosphate buffered saline (pH7.4) and 10% glycerol.
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Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
GPNFPQIDTP NMLPAHHRQK RDWIWNQMHI DEEKNESLPH
YVGKIKSNVN RQNAKYVLQG EFAGKIFGVD ANTGNVLAYE RLDREKVSEY FLTALIVDKN TNKNLEQPSS
FTVKVHDIND NWPVFSHQVF NASVPEMSAI GTSVIRVTAV DADDPTVAGH ATVLYQIVKG NEYFSIDNSG
LIFTKIKNLD REKQAEYKIV VETQDALGLR GESGTATVMI RLEDINDNFP VFTQSTYTFS VPEDIRVGKP
LGFLTVVDPD EPQNRMTKYS IMQGEYRDTF TIETDPKRNE GIIKPTKSLD YEVIQQYTFY IEATDPTIRY
EYLSSTSGKN KAMVTINVLD VDEPPVFQRH FYHFKLPENQ KKPLIGTVVA KDPDKAQRSI GYSIRKTSDR
GQFFRITKQG NIYNEKELDR ETYAWYNLTV EANELDSRGN PVGKESIVQV YIEVLDENDN PPEFAQPYEP
KVCENAAQGK LVVQISATDK DVVPVNPKFK FALKNEDSNF TLINNHDNTA NITVKYGQFN REHAKFHYLP
VLISDNGVPS LTGTSTLTVG VCKCNEQGEF TFCEEMAAQA GVSIQLEHHH HHH.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
