Catalogue number
PRO-2122
Synonyms
Bromodomain Containing 2, RING3, Really Interesting New
Gene 3 Protein, Bromodomain-Containing 2, O27.1.1, FSRG1, Female Sterile Homeotic-Related
Gene 1, Bromodomain-Containing Protein 2, KIAA9001, D6S113E, RNF3, FSH, NAT,
Bromodomain-containing protein 2 .
Introduction
Bromodomain Containing 2, also known as BRD2 is a transcriptional
regulator which is a member of the BET, bromodomains and extra terminal domain, family of
roteins. BRD2 links with transcription complexes and it acetylated chromatin in
the course of mitosis, and it selectively binds o the acetylated lysine-12 residue of
histone H4 through its two romodomains. BRD2 maps to the major
histocompatability complex MHC) class II
region on chromosome 6p21.3, however sequence comparison
advocate that the protein is not implicated in the immune response. BRD2
has been implicated in juvenile
myoclonic epilepsy, a common form of epilepsy which grows to be visible in
adolescence.
Description
BRD2 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 478 amino acids (1-455 a.a) and
having a molecular mass of 52.8kDa.
BRD2 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
BRD2 protein solution (1mg/ml) containing Phosphate
buffered saline (pH7.4), 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMLQNVTP HNKLPGEGNA GLLGLGPEAA APGKRIRKPS LLYEGFESPT
MASVPALQLT PANPPPPEVS NPKKPGRVTN QLQYLHKVVM KALWKHQFAW PFRQPVDAVK LGLPDYHKII
KQPMDMGTIK RRLENNYYWA ASECMQDFNT MFTNCYIYNKPTDDIVLMAQ TLEKIFLQKV ASMPQEEQEL VVTIPKNSHK KGAKLAALQG
SVTSAHQVPA VSSVSHTALY TPPPEIPTTV LNIPHPSVIS SPLLKSLHSA GPPLLAVTAA PPAQPLAKKK
GVKRKADTTT PTPTAILAPG SPASPPGSLE PKAARLPPMR RESGRPIKPP RKDLPDSQQQHQSSKKGKLS EQLKHCNGIL KELLSKKHAA YAWPFYKPVD ASALGLHDYH
DIIKHPMDLS TVKRKMENRD YRDAQEFAAD VRLMFSNCYK YNPPDHDVVA MARKLQDVFE FRYAKMPD.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
