Catalogue number
IHA-035
Introduction
Influenza A virus subtype H5N1 is a subtype of the Influenza A virus. Subtype H5N1 is commonly known as avian influenza or bird flu. H5N1 may cause more than one influenza pandemicas it is expected to continue mutating in birds. The dominant strain of HPAI A (H5N1) evolved creating the Z genotype. It has also been called Asian lineage HPAI/A/H5N1 which is divided into 2 antigenic clades. "Clade 1 includes human and bird isolates from Vietnam, Thailand, and Cambodia and bird isolates from Laos and Malaysia. Clade 2 viruses were first identified in bird isolates from China, Indonesia, Japan, and South Korea before spreading westward to the Middle East, Europe, and Africa.
Description
Recombinant Vietnam HN31242/2007 produced in Hi-5 cell of Baculovirus is a single polypeptide chain containing 332 amino acids (17-338) and having a molecular mass of 37.4kDa.
H5N1 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Source
Baculovirus
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The H5N1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
ADPMDQICIG YHANNSTEQV DTIMEKNVTV THAQDILEKT HNGKLCDLDG VKPLILRDCS VAGWLLGNPM CDEFINVPEW SYIVEKANPA NDLCYPGNFN DYEELKHLLS RINHFEKIQI IPKSSWSDHE ASSGVSSACP YQGVPSFFRN VVWLIKKNNT YPTIKRSYNN TNQEDLLILW GIHHSNDAAE QTKLYQNPTT YISVGTSTLN QRLVPKIATR SKVNGQSGRM DFFWTILKPN DAINFESNGN FIAPEYAYKI VKKGDSAIMK SEVEYGNCNT KCQTPIGAIN SSMPFHNIHP LTIGECPKYV KSNKLVLATG LRNSPLHHHH HH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
