Catalogue number
PRO-1738
Synonyms
Synaptotagmin V, Synaptotagmin 5, synaptotagmin-5, sytV, SytV.
Introduction
Synaptotagmin
V, (SYT5) is a member of synaptotagmin family, which is a family of type III
membrane proteins characterized by cytoplasmic repeats related to protein
kinase C regulatory (C2) domains that are considered to bind calcium.
Synaptotagmins function as negative regulators of vesicle fusion, allowing
fusion in the attendance of calcium, and as calcium receptors or sensor
molecules. Among the diseases associated with SYT5 are labyrinthitis, and
thyroiditis.
Description
SYT5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 301 amino acids (109-386aa) and having a molecular mass of 33.6kDa.
SYT5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SYT5 protein solution (0.25mg/ml) containing 20mM
Tris-HCl(pH 8.0) containing 40% glycerol, 0.2M NaCl and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSLGRLQYS LDYDFQSGQL LVGILQAMGL AALDLGGSSD PYVRVYLLPD
KRRRYETKVH RQTLNPHFGE TFAFKVPYVE LGGRVLVMAV YDFDRFSRND AIGEVRVPMS SVDLGRPVQA
WRELQAAPRE EQEKLGDICF SLRYVPTAGK LTVIVLEAKN LKKMDVGGLS DPYVKVHLLQ GGKKVRKKKT TIKKNTLNPY YNEAFSFEVP
CDQVQKVQVE LTVLDYDKLG KNEAIGRVAV GAAAGGAGLR HWADMLANPR RPIAQWHSLR PPDRVRLLPA P
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
