Catalogue number
PKA-085
Synonyms
Receptor
tyrosine-protein kinase erbB-3, Glial growth factor receptor, Proto-oncogene-like
protein c-ErbB-3.
Introduction
ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 can induce specific antibody production in vivo, hence to inhibit tumor cell growth. ErbB-3 can be used to treat early, medium and advanced or post-operative breast cancer with over-expression of ErbB2. According to its mechanism of action, ErbB3 is classified as a therapeutic for cancer.
Description
ErbB3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 630 amino acids (20-641.a.) and having a molecular mass of 69.5kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
ErbB3 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
ErbB3 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
SEMGNSQAVC PGTLNGLSVT GDADNQYQTL YKLYEKCEVV MGNLEIVLTG
HNADLSFLQW IREVTGYVLV AMNEFSVLPL PNLRVVRGTQ VYDGKFAIFV MLNYNTNSSH ALRQLRFTQL
TEILLGGVYI EKNDKLCHMD TIDWRDIVRV PDAEIVVKNN GGNCPPCHEV CKGRCWGPGP
EDCQILTKTI CAPQCNGRCF GPNPNQCCHD ECAGGCSGPQ DTDCFACRHF
NDSGACVPRC PAPLVYNKLT FQLEPNPHIK YQYGGVCVAS CPHNFVVDQT FCVRACPADK MEVDKNGLKM
CEPCRGLCPK ACEGTGSGSR YQTVDSSNID GFVNCTKILG NLDFLITGLN GDPWHKIPAL
DPEKLNVFRT VREITGYLNI QSWPPHMHNF SVFSNLTTIG GRSLYNRGFS
LLIMKNLNVT SLGFRSLKEI SAGRVYISAN QQLCYHHSLN WTRLLRGPAE ERLDIKYNRP LGECVAEGKV
CDPLCSSGGC WGPGPGQCLS CRNYSREGVC VTHCNVLQGE PREFVHEAHC FSCHPECQPM
EGTSTCNGSG SDACARCAHF
RDGPHCVNSC PHGILGAKGP IYKYPDAQNE CRPCHENCTQ GCKGPELQDC LGQAEVLMSK PHLEHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
