Catalogue number
ENZ-880
Synonyms
X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble,
XPNPEPL, SAMP, X-Prolyl Aminopeptidase 1, Soluble, Aminoacylproline
Aminopeptidase, Cytosolic Aminopeptidase P, Soluble Aminopeptidase P, X-Pro
Aminopeptidase 1, EC 3.4.11.9, XPNPEPL1, X-Prolyl Aminopeptidase
(Aminopeptidase P)-Like, Aminopeptidase P, Cytosolic, Xaa-Pro Aminopeptidase 1,
XPNPEP, APP1, Xaa-Pro aminopeptidase 1.
Introduction
X-Prolyl Aminopeptidase-1, also known as XPNPEP1 is
a member of the peptidase M24B family. XPNPEP1 encodes the cytosolic form of a
metalloaminopeptidase which catalyzes the cleavage of the N-terminal amino acid
adjacent to a proline residue. Furthermore, XPNPEP1 plays a role in degradation
as well as maturation of tachykinins, neuropeptides and peptide hormones.
Description
XPNPEP1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 655 amino acids (1-623 a.a) and
having a molecular mass of 73.4kDa.
XPNPEP1 is fused to a 32 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
XPNPEP1 protein solution (1mg/ml) containing Phosphate
buffered saline (pH7.4) and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSEFELRRQ ASMPPKVTSE LLRQLRQAMR NSEYVTEPIQ AYIIPSGDAH QSEYIAPCDC
RRAFVSGFDG SAGTAIITEE HAAMWTDGRY FLQAAKQMDS NWTLMKMGLK DTPTQEDWLV SVLPEGSRVG
VDPLIIPTDY WKKMAKVLRS AGHHLIPVKE NLVDKIWTDR PERPCKPLLT LGLDYTGISW KDKVADLRLK
MAERNVMWFV VTALDEIAWL FNLRGSDVEH NPVFFSYAII GLETIMLFID GDRIDAPSVK EHLLLDLGLE
AEYRIQVHPY KSILSELKAL CADLSPREKV WVSDKASYAV SETIPKDHRC CMPYTPICIA KAVKNSAESE
GMRRAHIKDA VALCELFNWL EKEVPKGGVT EISAADKAEE FRRQQADFVD LSFPTISSTG PNGAIIHYAP
VPETNRTLSL DEVYLIDSGA QYKDGTTDVT RTMHFGTPTA YEKECFTYVL KGHIAVSAAV FPTGTKGHLL
DSFARSALWD SGLDYLHGTG HGVGSFLNVH EGPCGISYKT FSDEPLEAGM IVTDEPGYYE DGAFGIRIEN
VVLVVPVKTK YNFNNRGSLT FEPLTLVPIQ TKMIDVDSLT DKECDWLNNY HLTCRDVIGK ELQKQGRQEA
LEWLIRETQP ISKQH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
