Catalogue number
PKA-216
Synonyms
Mitogen-activated protein kinase 9, EC 2.7.11.24, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, JNK-55, JNK2, JNK2A, JNK2B, PRKM9, JNK2BETA, p54aSAPK, JNK2ALPHA, MAPK9, SAPK1.
Introduction
JUN N-terminal Kinase 2is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Description
SAPK1/JNK2 Human Recombinant (stress-activated protein kinase/c-jun kinase) is a non-glycosilated polypeptide having a molecular mass of 49.5 kDa.
SAPK1/JNK2 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
JNK2/SAPK1 (1.3 mg/ml) is supplied in 50mM Tris-HCl, 100mM NaCl, 1mM DTT, 50% glycerol, pH 8.
Unit Definition
No protease activity detectable, specific activity > 300 U*/mg (*1 U = 1 pmol/min transferred to c-jun (1-169) at 30°C). Autophosphorylation < 5%.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
