Catalogue number
PKA-050
Synonyms
Serine/threonine-protein kinase 17B, EC 2.7.11.1, DAP kinase-related apoptosis-inducing protein kinase 2, STK17B, DRAK2.
Introduction
Serine/threonine kinase 17b (STK17B) is a member of the protein kinase superfamily. STK17B functions as a positive regulator of apoptosis. STK17B interacts with CHP1; this interaction stimulates CHP1 to translocate from the Golgi to the nucleus. STK17B is highly expressed in the placenta, lung, pancreas, however it has lower levels in the heart, brain, liver, skeletal muscle and kidney.
Description
STK17B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 395 amino acids (1-372 a.a) and having a molecular mass of 44.7kDa.
STK17B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
STK17B protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSRRRFD CRSISGLLTT TPQIPIKMEN FNNFYILTSK ELGRGKFAVV RQCISKSTGQ EYAAKFLKKR RRGQDCRAEI LHEIAVLELA KSCPRVINLH EVYENTSEII LILEYAAGGE IFSLCLPELA EMVSENDVIR LIKQILEGVY YLHQNNIVHL DLKPQNILLS SIYPLGDIKI VDFGMSRKIG HACELREIMG TPEYLAPEIL NYDPITTATD MWNIGIIAYM LLTHTSPFVG EDNQETYLNI SQVNVDYSEE TFSSVSQLAT DFIQSLLVKN PEKRPTAEIC LSHSWLQQWD FENLFHPEET SSSSQTQDHS VRSSEDKTSK SSCNGTCGDR EDKENIPEDS SMVSKRFRFD DSLPNPHELV SDLLC.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
