Catalogue number
PKA-068
Synonyms
V-Akt Murine Thymoma Viral Oncogene Homolog 1, Protein
Kinase B Alpha, Proto-Oncogene C-Akt, RAC-PK-Alpha, EC 2.7.11.1, PKB Alpha, CWS6,
PKB, RAC, RAC-Alpha Serine/Threonine-Protein Kinase, Rac Protein Kinase Alpha,
Protein Kinase B, PKB-ALPHA, RAC-ALPHA, EC 2.7.11, AKT1m, PRKBA, AKT, RAC-alpha
serine/threonine-protein kinase.
Introduction
Akt1, also known as Akt or else protein kinase B (PKB) is an important
molecule in mammaliancellular signaling. In humans, there are three genes in
the "Akt family": Akt1, Akt2, and Akt3. Moreover, these enzymes are
members of the serine/threonine-specific protein kinase family (EC2.7.11.1). Akt1
is involved in cellular survival pathways, by inhibiting apoptoticprocesses.
Akt1 is also able to induce protein synthesis pathways, and is therefore a key
signaling protein in the cellular pathways which lead to skeletal muscle
hypertrophy, and general tissue growth. Since it can block apoptosis, and
thereby promote cell survival, Akt1 has been implicated as a most important
factor in numerous types of cancer. Akt (now also called Akt1) was at first
identified as the oncogenein the transforming retrovirus, AKT8.
Description
AKT1 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 503 amino acids (1-480 a.a) and having a molecular
mass of 58.1kDa.
AKT1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
AKT1
protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSDVAIV KEGWLHKRGE YIKTWRPRYF LLKNDGTFIG YKERPQDVDQ
REAPLNNFSV AQCQLMKTER PRPNTFIIRC LQWTTVIERT FHVETPEERE EWTTAIQTVA DGLKKQEEEE
MDFRSGSPSD NSGAEEMEVS LAKPKHRVTM NEFEYLKLLGKGTFGKVILV KEKATGRYYA MKILKKEVIV AKDEVAHTLT ENRVLQNSRH
PFLTALKYSF QTHDRLCFVM EYANGGELFF HLSRERVFSE DRARFYGAEI VSALDYLHSE KNVVYRDLKL
ENLMLDKDGH IKITDFGLCK EGIKDGATMK TFCGTPEYLA PEVLEDNDYG RAVDWWGLGVVMYEMMCGRL PFYNQDHEKL FELILMEEIR FPRTLGPEAK SLLSGLLKKD
PKQRLGGGSE DAKEIMQHRF FAGIVWQHVY EKKLSPPFKP QVTSETDTRY FDEEFTAQMI TITPPDQDDS
MECVDSERRP HFPQFSYSAS GTA.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
