Catalogue number
ENZ-957
Synonyms
Folh1, GCP2, mopsm, Glutamate carboxypeptidase 2, Folate
hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate
carboxypeptidase II, GCPII, Naalad1.
Introduction
Folate Hydrolase 1 (Folh1) is a
single pass type 2 membrane protein which is expressed mainly in prostate epithelium.
Folh1 which is a part of the peptidase M28 family and M28B subfamily has both
folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase activity. Folh1
can be found in urinary bladder, kidney, testis, ovary, stomach, small
intestine colon, and the capillary endothelium of various tumors. Therefore, Folh1
plays a role in directed imaging and therapy of recurrent of metastatic
disease.
Description
FOLH1 Mouse Recombinant produced in in Sf9 Baculovirus
cells is a single, non-glycosylated polypeptide chain containing 717 amino
acids (45-752a.a) and having a molecular mass of 80.5kDa (Migrates at 70-100kDa
on SDS-PAGE under reducing conditions). FOLH1 is fused to a 6 amino acid His-tag at C-Terminus
and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The FOLH1 solution (0.5mg/ml) contains Phosphate Buffered
Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
ADPKPSNEAT GNVSHSGMKK EFLHELKAEN IKKFLYNFTR TPHLAGTQNN
FELAKQIHDQ WKEFGLDLVE LSHYDVLLSY PNKTHPNYIS IINEDGNEIF KTSLSEQPPP GYENISDVVP
PYSAFSPQGT PEGDLVYVNY ARTEDFFKLE REMKISCSGK IVIARYGKVF RGNMVKNAQL AGAKGMILYS
DPADYFVPAV KSYPDGWNLP GGGVQRGNVL NLNGAGDPLT PGYPANEHAY RHELTNAVGL PSIPVHPIGY
DDAQKLLEHM GGPAPPDSSW KGGLKVPYNV GPGFAGNFST QKVKMHIHSY TKVTRIYNVI GTLKGALEPD
RYVILGGHRD AWVFGGIDPQ SGAAVVHEIV RSFGTLKKKG RRPRRTILFA SWDAEEFGLL GSTEWAEEHS
RLLQERGVAY INADSSIEGN YTLRVDCTPL MYSLVYNLTK ELQSPDEGFE GKSLYDSWKE KSPSPEFIGM
PRISKLGSGN DFEVFFQRLG IASGRARYTK NWKTNKVSSY PLYHSVYETY ELVVKFYDPT FKYHLTVAQV
RGAMVFELAN SIVLPFDCQS YAVALKKYAD TIYNISMKHP QEMKAYMISF DSLFSAVNNF
TDVASKFNQR LQELDKSNPI LLRIMNDQLM YLERAFIDPL
GLPGRPFYRH IIYAPSSHNK YAGESFPGIY DALFDISSKV NASKAWNEVK RQISIATFTV QAAAETLREV AHHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
