Catalogue number
ENZ-543
Synonyms
WISP39, DIR1, NG7, FK506-binding protein-like, WAF-1/CIP1 stabilizing protein 39, FKBPL.
Introduction
FKBPL is related to the immunophilin protein family, which take part in immunoregulation and regular cellular processes involving protein folding and trafficking. FKBPL has an important function in the induced radioresistance and participates in the control of the cell cycle. FKBPL has a role in cellular response to stress. FKBPL interacts with Hsp90, glucocorticoid receptor and dynamitin and is involved in signalling, like other FKBPs.
Description
FKBPL Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 357 amino acids (1-349 a.a.) and having a molecular mass of 39.2 kDa. The FKBPL is fused to an 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
FKBPL Human solution containing 20mM Trsi pH-8, 2mM DTT, 0.1M NaCl & 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Biological Activity
Specific activity is > 210 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
Amino acid sequence
METPPVNTIG EKDTSQPQQE WEKNLRENLD SVIQIRQQPR DPPTETLELE VSPDPASQIL EHTQGAEKLV AELEGDSHKS HGSTSQMPEA LQASDLWYCP DGSFVKKIVI RGHGLDKPKL GSCCRVLALG FPFGSGPPEG WTELTMGVGP WREETWGELI EKCLESMCQG EEAELQLPGH SGPPVRLTLA SFTQGRDSWE LETSEKEALA REERARGTEL FRAGNPEGAA RCYGRALRLL LTLPPPGPPE RTVLHANLAA CQLLLGQPQL AAQSCDRVLE REPGHLKALY RRGVAQAALG NLEKATADLK KVLAIDPKNR AAQEELGKVV IQGKNQDAGL AQGLRKMFGL EHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
