Catalogue number
PKA-052
Synonyms
Mevalsonate Kinase (Mevalsonic Aciduria), LH Receptor MRNA-Binding Protein, LRBP, Mevalsonate Kinase 1, MK, MVLK, EC 2.7.1.36.
Introduction
MVK is a member of the GHMP kinase family. Mevalsonate is a vital mediator, and MVK an important early enzyme, in isoprenoid and sterol synthesis. MVK deficiency can result in gene mutation associated with Mevalsonic aciduria, an illness which causes psychomotor retardation, failure to thrive hepatosplenomegaly, anemia and frequent febrile crises. Mutations in this gene also cause hyperimmunoglobulinaemia D and sporadic fever syndrome, in which the person suffers from recurrent episodes of fever related with lymphadenopathy, skin rash, gastrointestinal dismay and arthralgia.
Description
MVK Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 419 amino acids (1-396) and having a molecular mass of 44.8kDa.
The MVK is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
MVK protein (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMLSEVLL VSAPGKVILH GEHAVVHGKV ALAVSLNLRT FLRLQPHSNG KVDLSLPNIG IKRAWDVARL QSLDTSFLEQ GDVTTPTSEQ VEKLKEVAGL PDDCAVTERL AVLAFLYLYL SICRKQRALP SLDIVVWSEL PPGAGLGSSA AYSVCLAAAL LTVCEEIPNP LKDGDCVNRW TKEDLELINK WAFQGERMIH GNPSGVDNAV STWGGALRYH QGKISSLKRS PALQILLTNT KVPRNTRALV AGVRNRLLKF PEIVAPLLTS IDAISLECER VLGEMGEAPA PEQYLVLEEL IDMNQHHLNA LGVGHASLDQ LCQVTRARGL HSKLTGAGGG GCGITLLKPG LEQPEVEATK QALTSCGFDC LETSIGAPGV SIHSATSLDS RVQQALDGL.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
