Catalogue number
ANT-292
Synonyms
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4, CD4mut.
Type
Rat Anti Mouse Monoclonal.
Introduction
CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized.
Clone
mCD4.
Immunogen
Purified mouse LN CD4+ T cells.
Ig Subclass
Rat IgG.
Purification Method
Ion exchange column.
Formulation
1mg/ml in PBS (after reconstitution).
Storage Procedures
Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Solubility
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Applications
FACS staining, immunohistochemistry, Western Blotting. For staining, use 10 µl/1,000,000 cells.
Available Conjugates
This antibody is also available unconjugated and conjugated to biotin.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
