Catalogue number
ANT-046
Synonyms
Hypoxanthine-Guanine Phosphoribosyltransferase, EC 2.4.2.8, HGPRT, HGPRTase, HPRT, HPRT1.
Type
Mouse Anti Human Monoclonal.
Introduction
HPRT1 has a main part in the generation of purine nucleotides through the purine salvage pathway. HPRT1 primarily functions to salvage purines from degraded DNA to renewed purine synthesis. Therefore, it performs as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.
Clone
PAT2G8AT.
Immunogen
Anti-human HPRT mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HPRT amino acids 1-218 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Purification Method
HPRT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
HPRT antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500~1:5000.
Recommended starting dilution is 1:1000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
