Catalogue number
PRO-1015
Synonyms
U2 small nuclear ribonucleoprotein A', U2 snRNP A', SNRPA1, Lea1.
Introduction
U2 small nuclear ribonucleoprotein A (SNRPA1), a 255 amino acid protein, is a component of the U2 snRNP which forms a complex with U2 snRNP B (U2B). U2 snRNP A and U2 snRNP B form together a complex which binds to the U2 snRNA hairpin IV. The configuration of the U2 snRNP A/U2 snRNP B dimer and the delicate variations of a few key residues regulate the snRNP-RNA-binding specificity.
Description
SNRPA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 275 amino acids (1-255 a.a.) and having a molecular mass of 30.5kDa.
SNRPA1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SNRPA1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.2M NaCl,2mM DTT and 0.1mM PMSF.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEK MFKGKRGAQL AKDIARRSKT FNPGAGLPTD KKKGGPSPGD VEAIKNAIAN ASTLAEVERL KGLLQSGQIP GRERRSGPTD DGEEEMEEDT VTNGS.
Safety Data Sheet
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
