Catalogue number
PRO-1907
Synonyms
CD1A, R2, T-cell surface glycoprotein CD1e, membrane-associated, R2G1, sCD1e.
Introduction
CD1E belongs to the CD1
family of transmembrane glycoproteins which are structurally associated to the main
MHC (histocompatibility complex) proteins and form heterodimers with
beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid
and glycolipid antigens of self or microbial origin to T cells. The human
genome is comprised of 5 CD1 family genes organized in a cluster on chromosome
1. The CD1 family members are belief to differ in their cellular localization
and specificity for particular lipid ligands. CD1E localizes within Golgi
compartments, endosomes, and lysosomes, and is cleaved into a steady soluble
form. The soluble form is necessary for the intracellular processing of some
glycolipids into a form that can be presented by other CD1 family members.
Description
CD1E Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 297 amino acids (32-305 a.a) and
having a molecular mass of 33.1kDa.CD1E is fused to a 23 amino acid His-tag at N-terminus &
purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CD1E protein solution (1mg/ml) contains 20mM Tris-HCl buffer
(pH 8.0), 10% glycerol and 0.4M Urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSEEQLSFR MLQTSSFANH SWAHSEGSGW LGDLQTHGWD TVLGTIRFLK
PWSHGNFSKQ ELKNLQSLFQ LYFHSFIQIV QASAGQFQLE YPFEIQILAG CRMNAPQIFL NMAYQGSDFL
SFQGISWEPS PGAGIRAQNI CKVLNRYLDI KEILQSLLGH TCPRFLAGLM EAGESELKRK VKPEAWLSCG
PSPGPGRLQL VCHVSGFYPK PVWVMWMRGE QEQRGTQRGD VLPNADETWY LRATLDVAAG EAAGLSCRVK
HSSLGGHDLI IHWGGYS.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
