Catalogue number
PRO-2071
Synonyms
SWI/SNF Related, Matrix Associated, Actin Dependent
Regulator Of Chromatin, Subfamily A, Member 4, Mitotic Growth And Transcription
Activator, ATP-Dependent Helicase SMARCA4, BRG1, Global Transcription Activator
Homologous Sequence
Sucrose Nonfermenting-Like 4, BRG1-Associated Factor 190A,
Protein Brahma Homolog 1, BRM/SWI2-Related Gene 1, Homeotic Gene Regulator, Brahma
Protein-Like 1
Nuclear Protein GRB1, Protein BRG-1, SNF2-Like 4, SNF2-Beta,
BAF190A, SNF2L4, MRD16, RTPS2, SWI/SNF-Related Matrix-Associated
Actin-Dependent Regulator Of Chromatin Subfamily A Member 4, Transcription
Activator BRG1, EC 3.6.4.-, EC 3.6.1, BAF190, SNF2LB, HSNF2b, SNF2B, SNF2, SWI2,
Transcription activator BRG1.
Introduction
SMARCA4 belongs to the SWI/SNF family of proteins and is similar to the
brahma protein of Drosophila.
Proteins in this family have helicase and ATPase activities and are considered
to regulate transcription of
certain genes by altering the chromatin structure around those genes. SMARCA4
is part of the large ATP-dependent chromatin
remodeling complex SNF/SWI, which is essential for transcriptional activation of genes normally repressed by chromatin. In
addition, SMARCA4 is able to bind BRCA1, as well as regulating the expression
of the
tumorigenic protein CD44. Moreover, mutations in SMARCA4 cause rhabdoid tumor
predisposition syndrome type 2.
Description
SMARCA4 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 211 amino acids (362-549aa) and
having a molecular mass of 25kDa.
SMARCA4 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SMARCA4 protein solution (1mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSDPVEILQ EREYRLQARI AHRIQELENL PGSLAGDLRT KATIELKALR
LLNFQRQLRQ EVVVCMRRDT ALETALNAKA YKRSKRQSLR EARITEKLEK QQKIEQERKR RQKHQEYLNS
ILQHAKDFKE YHRSVTGKIQ KLTKAVATYH ANTEREQKKE
NERIEKERMR RLMAEDEEGY RKLIDQKKDK R.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
