Catalogue number
PRO-2127
Synonyms
bA397G5.3, C6orf51, TFIIIC35, General transcription
factor 3C polypeptide 6, Transcription factor IIIC 35 kDa subunit, TFIIIC 35
kDa subunit, TFIIIC35, Transcription factor IIIC subunit 6, CDA020, NPD020.
Introduction
In the absence of additional proteins - the general transcription factors (GTFs) RNA polymerases are unable to initiate RNA synthesis. GTFs accumulate in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins are vital for RNA polymerase III to produce several small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin.
Description
GTF3C6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (1-213 a.a) and having a molecular mass of 26.4kDa.
GTF3C6 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
GTF3C6 protein solution (0.5mg/ml) containing PBS buffer
(pH 7.4), 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAAADE RSPEDGEDEE EEEQLVLVEL SGIIDSDFLS KCENKCKVLG IDTERPILQV
DSCVFAGEYE DTLGTCVIFE ENVEHADTEG NNKTVLKYKC HTMKKLSMTR TLLTEKKEGE ENIGGVEWLQ
IKDNDFSYRP NMICNFLHEN EDEEVVASAP DKSLELEEEE IQMNDSSNLS CEQEKPMHLE IEDSGPLIDI
PSETEGSVFM ETQMLP.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
