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Collagen Triple Helix Repeat Containing 1, Protein NMTC1, CTHRC1.

Collagen triple helix repeat-containing protein 1 (CTHRC1) functions as a negative regulator of collagen matrix deposition. CTHRC1 is a secreted 28kDa protein which is glycosylated and highly conserved from lower chordates to mammals. CTHRC1 is highly connected with calcified tissues and cartilaginous matrix, but not with endothelial cells. CTHRC1 is detected qualitatively in plasma of healthy human subjects. CTHRC1 plasma levels are also significantly elevated during pregnancy, in diabetes, in inflammatory and infectious conditions, in subjects with acute myeloid leukemia but not in subjects with solid cancers. The hormonal functions of CTHRC1 include regulation of lipid storage and cellular glycogen levels with potentially far-reaching implications for cell metabolism and physiology. CTHRC1 gene deletion leads to fatty liver (steatosis) formation in mice while others exhibited inactivation of the CTHRC1 gene also results in low bone mass.

CTHRC1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (31-243 a.a) and having a molecular mass of 25.3kDa.
CTHRC1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile filtered colorless solution.

CTHRC1 protein solution (1mg/ml) containing 20mM Tris-HCl (pH 8.0) and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Greater than 90.0% as determined by SDS-PAGE.

MGSSHHHHHH SSGLVPRGSH MSEIPKGKQK AQLRQREVVD LYNGMCLQGP AGVPGRDGSP GANGIPGTPG IPGRDGFKGE KGECLRESFE ESWTPNYKQC SWSSLNYGID LGKIAECTFT KMRSNSALRV LFSGSLRLKC RNACCQRWYF TFNGAECSGP LPIEAIIYLD QGSPEMNSTI NIHRTSSVEG LCEGIGAGLV DVAIWVGTCS DYPKGDASTG WNSVSRIIIE ELPK.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.