Catalogue number
ENZ-949
Synonyms
Tyrosylprotein Sulfotransferase 2, EC 2.8.2.20, TPST-2,
Transport And Golgi Organization 13 Homolog B (Drosophila), Transport And Golgi
Organization 13 Homolog B, Protein-Tyrosine Sulfotransferase 2, Tyrosylprotein
Phosphotransferase 2, Tyrosylprotein Sulfotransferase-2, TANGO13B.
Introduction
Tyrosylprotein Sulfotransferase 2 (TPST2) is a member of the protein sulfotransferase family. TPST2 is a widely expressed protein, which catalyzes the O-sulfation of tyrosine residues within acidic regions of proteins. The TPST2 protein is a type II integral membrane protein located in the Golgi body.
Description
TPST2 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 361 amino acids (26-377 a.a.) and
having a molecular mass of 40.4kDa (Migrates at 40-57kDa on SDS-PAGE under
reducing conditions).
TPST2 is expressed with a 6
amino acid His tag at C-Terminus and purified by proprietary chromatographic
techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
TPST2 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
ADPQQVLECR AVLAGLRSPR GAMRPEQEEL VMVGTNHVEY RYGKAMPLIF
VGGVPRSGTT LMRAMLDAHP EVRCGEETRI IPRVLAMRQA WSKSGREKLR LDEAGVTDEV LDAAMQAFIL
EVIAKHGEPA RVLCNKDPFT LKSSVYLSRL FPNSKFLLMV RDGRASVHSM ITRKVTIAGF DLSSYRDCLT
KWNKAIEVMY AQCMEVGKEK CLPVYYEQLV LHPRRSLKLI LDFLGIAWSD AVLHHEDLIG KPGGVSLSKI
ERSTDQVIKP VNLEALSKWT GHIPGDVVRD MAQIAPMLAQ LGYDPYANPP NYGNPDPFVI NNTQRVLKGD
YKTPANLKGY FQVNQNSTSS HLGSSHHHHH H.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
