Catalogue number
PRO-2072
Synonyms
Potassium Channel Subfamily M Regulatory Beta Subunit 3, Potassium
Large Conductance Calcium-Activated Channel, Subfamily M Beta Member 3, Calcium-Activated
Potassium Channel, Subfamily M Subunit Beta-3, Charybdotoxin Receptor Subunit
Beta-3, Big Potassium Channel Beta Subunit 3, Maxi K Channel Subunit Beta-3, MaxiK Channel Beta-Subunit 3, BK Channel Beta Subunit 3, BK
Channel Subunit Beta-3, K(VCA)BETA-3, SLO-BETA-3,
BKBETA3, Hbeta3, KCNMB2, KCNMBL, Large Conductance, Voltage And Ca2+ Activated
Potassium Channel Maxi K Beta 3 Subunit, Calcium-Activated Potassium Channel
Regulatory Subunit, SLOBETA3, Calcium-activated potassium channel subunit
beta-3.
Introduction
The KCNMB3 belongs to a family of 4 auxiliary beta subunits found in the mammalian genome, which associate with Slo1 alpha subunits and regulate BK channel function. In humans, the KCNMB3 gene is comprised of 4 N-terminal alternative exons which produce 4 functionally distinct beta3 subunits, beta3a-d. Three variants, beta3a-c, display kinetically distinct inactivation behaviors.
Description
KCNMB3 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 149 amino acids (82-207 a.a) and
having a molecular mass of 16.8kDa.
KCNMB3 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
KCNMB3
protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA
and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSKPFMLSI QREESTCTAI HTDIMDDWLD CAFTCGVHCH GQGKYPCLQV
FVNLSHPGQK ALLHYNEEAV QINPKCFYTP KCHQDRNDLL NSALDIKEFF DHKNGTPFSC FYSPASQSED
VILIKKYDQ.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
