Catalogue number
ENZ-973
Synonyms
B3GNT2, B3GN-T2, B3GNT, B3GNT-2, B3GNT1, BETA3GNT,
BGnT-2, BGNT2, N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
2, Beta-1,3-N-acetylglucosaminyltransferase 1, BGnT-1, Beta-1,3-Gn-T1,
Beta3Gn-T1, Beta-1,3-galactosyltransferase 7, Beta-1,3-GalTase 7, Beta3Gal-T7,
Beta3GalT7,
b3Gal-T7, Beta-3-Gx-T7, beta-GlcNAc
beta-1,3-galactosyltransferase 7, betaGal
beta-1,3-N-acetylglucosaminyltransferase 2, BGnT-2, Beta-1,3-Gn-T2,
Beta-1,3-N-acetylglucosaminyltransferase 2, Beta3Gn-T2,
beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7.
Introduction
Beta-1,3-N-Acetylglucosaminyltransferase
2 (B3GNT2) is a part of the beta-1,3-N-acetylglucosaminyltransferase family
which takes part in the synthesis of poly-N-acetyllactosamine. B3GNT2 is a type II transmembrane protein
which prefers the substrate of lacto-N-neotetraose. B3GNT2 catalyzes the
initiation and elongation of poly-N-acetyllactosamine chains and comprises the
main polylactosamine synthase.
Description
B3GNT2 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 375 amino acids (29-397a.a.) and having
a molecular mass of 43.5kDa (Molecular size on SDS-PAGE will appear at
approximately 40-57kDa).
B3GNT2 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
B3GNT2 protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPKSSSQEK
NGKGEVIIPK EKFWKISTPP EAYWNREQEK LNRQYNPILS MLTNQTGEAG RLSNISHLNY CEPDLRVTSV
VTGFNNLPDR FKDFLLYLRC RNYSLLIDQP DKCAKKPFLL LAIKSLTPHF ARRQAIRESW GQESNAGNQT
VVRVFLLGQT PPEDNHPDLS DMLKFESEKH QDILMWNYRD TFFNLSLKEV LFLRWVSTSC PDTEFVFKGD
DDVFVNTHHI LNYLNSLSKT KAKDLFIGDV IHNAGPHRDK KLKYYIPEVV YSGLYPPYAG GGGFLYSGHL
ALRLYHITDQ VHLYPIDDVY TGMCLQKLGL VPEKHKGFRT FDIEEKNKNN ICSYVDLMLV HSRKPQEMID IWSQLQSAHL
KCHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
