Catalogue number
PRO-1648
Synonyms
CD59 Molecule Complement Regulatory Protein, CD59 Antigen P18-20, CD59 Glycoprotein, Surface Anitgen Recognized By Monoclonal Antibody 16.3A5, Membrane Attack Complex (MAC) Inhibition Factor, Lymphocytic Antigen CD59/MEM43, Human Leukocyte Antigen MIC11, Membrane Attack Complex Inhibition Factor, 20 KDa Homologous Restriction Factor, Membrane Inhibitor Of Reactive Lysis, MAC-Inhibitory Protein, T Cell-Activating Protein, Ly-6-Like Protein, Protectin, MIC11, MSK21, MEM43, MIN1/2/3, MIRL, MAC-IP, MACIF, HRF-20, G344, EJ16/30/32, 1F5.
Introduction
CD59 is a cell surface glycoprotein which regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. CD59 is an effective inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the compilation of this complex, thus inhibiting the incorporation of multiple copies of C9 into the complex, which is essential for osmolytic pore formation. In addition, CD59 has a role in signal transduction pathways in the activation of T cells. CD59 gene mutations cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction.
Description
CD59 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 100 amino acids (26-102) and having a molecular mass of 11.3kDa.
CD59 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
The CD59 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSLQCYNCP NPTADCKTAV NCSSDFDACL ITKAGLQVYN KCWKFEHCNF NDVTTRLREN ELTYYCCKKD LCNFNEQLEN.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
