Catalogue number
ENZ-519
Synonyms
TP53I3, PIG3, Quinone Oxidoreductase, tumor protein p53 inducible protein 3.
Introduction
TP53I3 participates in the generation of reactive oxygen species (ROS). TP53I3 has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. TP53I3 has low NADPH-dependent diamine reductase activity (in vitro). TP53I3 is localized to the cytoplasm and induced in primary, non-transformed and transformed cell cultures after exposure to genotoxic agents. TP53I3 microsatellite polymorphism is associated with differential susceptibility to cancer.
Description
TP53I3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 352 amino acids (1-332 a.a.) and having a molecular mass of 37.6 kDa. TP53I3 protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
1mg/ml solution containing 20mM Tris HCl pH-8, 0.1M NaCl & 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze thaw cycles.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MLAVHFDKPG GPENLYVKEV AKPSPGEGEV LLKVAASALN RADLMQRQGQ YDPPPGASNI LGLEASGHVAELGPGCQGHW KIGDTAMALL PGGGQAQYVT VPEGLLMPIP EGLTLTQAAA IPEAWLTAFQ LLHLVGNVQA GDYVLIHAGL SGVGTAAIQLTRMAGAIPLV TAGSQKKLQM AEKLGAAAGF NYKKEDFSEA TLKFTKGAGV NLILDCIGGS YWEKNVNCLA LDGRWVLYGL MGGGDINGPLFSKLLFKRGS LITSLLRSRD NKYKQMLVNA FTEQILPHFS TEGPQRLLPV LDRIYPVTEI QEAHKYMEAN KNIGKIVLEL PQ.
Safety Data Sheet
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
