Catalogue number
ENZ-876
Synonyms
TIMP Metallopeptidase Inhibitor 1, TIMP, EPA, Tissue
Inhibitor Of Metalloproteinases 1, Fibroblast Collagenase Inhibitor, Erythroid-Potentiating
Activity, Collagenase Inhibitor, TIMP-1, CLGI, Tissue Inhibitor Of
Metalloproteinase 1 (Erythroid Potentiating Activity, Collagenase Inhibitor), Erythroid
Potentiating Activity, Metalloproteinase Inhibitor 1, EPO, HCI, Metalloproteinase
inhibitor 1.
Introduction
TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocations to the nucleus and also inhibits apoptosis in B-cells. The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds. TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones.
Increased TIMP1 levels are connected with squamous cell laryngeal carcinoma. TIMP1 overexpression is linked to gastric cancer.
Description
TIMP1 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 190 amino acids (24-207a.a.) and
having a molecular mass of 21.5kDa. (Molecular size on SDS-PAGE will appear at
approximately 18-28kDa).
TIMP1 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
TIMP1
protein solution (0.2mg/ml) contains phosphate buffered saline (pH7.4).
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
