Catalogue number
ANT-231
Type
Mouse Anti Human Monoclonal Antibody.
Synonyms
Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD235a, GYPA, GPA, MN, MNS, GPSAT, GPErik, HGpMiV, HGpMiXI, HGpSta(C).
Introduction
Glycophorins A & B (GYPA &GYPB) are the main sialoglycoproteins of the human erythrocyte membrane which carry the antigenic determinants for the MN and Ss blood groups. Along with the M or N and S or s antigens which normally occur in all populations, approximately 40 related variant phenotypes were identified. These variants comprise all the variants of the Miltenberger complex and some isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. GYPA is significant for the function of SLC4A1 and is necessary for high activity of SLC4A1. GYPA is involved in translocations of SLC4A1 to the plasma membrane. GYPA is also a receptor for: the influenza virus, Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans and is also a receptor for Hepatitis A virus (HAV).
Clone
NYRhGlycophorinA.
Immunogen
r.Human GlycophorinA.
Ig Subclass
Mouse IgG1.
Purification Method
Protein A column.
Formulation
1mg/ml in PBS (after reconstitution).
Shipping Conditions
Antibody is shipped lyophilized at ambient temperature.
Storage Procedures
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Solubility
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Titer
By direct ELISA, 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
Safety Data Sheet
Applications
Western Blot, imunohistochemistry, flow cytometry. For WB use 1µg/ml of antibody. For flow cytometry use 5-10 µl per one million cells.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
