Catalogue number
PRO-1180
Synonyms
Tubulin folding cofactor C, tubulin-specific chaperone c, Tubulin-folding cofactor C, CFC.
Introduction
Tubulin folding cofactor C (TBCC) is a member of the TBCC family. TBCC has a role in the control of centrosome and Golgi apparatus positioning, with effects on cell shape and cell migration. Cofactor C is 1 of 4 proteins (cofactors A,D,E and C) engaged in the pathway leading to properly folded b-tubulin from folding intermediates. Cofactor E attaches to the cofactor D/beta-tubulin complex; their interaction with cofactor C subsequently causes the release of beta-tubulin polypeptides which are bound to the native state.
Description
TBCC Human Recombinant produced in E. coli is a single polypeptide chain containing 369 amino acids (1-346) and having a molecular mass of 41.7 kDa.
TBCC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The TBCC solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMESVSCS AAAVRTGDME SQRDLSLVPE RLQRREQERQ LEVERRKQKR QNQEVEKENS HFFVATFARE RAAVEELLER AESVERLEEA ASRLQGLQKL INDSVFFLAA YDLRQGQEAL ARLQAALAER RRGLQPKKRF AFKTRGKDAA SSTKVDAAPG IPPAVESIQD SPLPKKAEGD LGPSWVCGFS NLESQVLEKR ASELHQRDVL LTELSNCTVR LYGNPNTLRL TKAHSCKLLC GPVSTSVFLE DCSDCVLAVA CQQLRIHSTK DTRIFLQVTS RAIVEDCSGI QFAPYTWSYP EIDKDFESSG LDRSKNNWND VDDFNWLARD MASPNWSILP EEERNIQWD
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
