Catalogue number
PRO-2251
Synonyms
Renin-1, Angiotensinogenase, Kidney renin, Ren1, Ren,
Ren-A, Ren1c, Ren1d, Rn-1, Rnr.
Introduction
Renin is a highly specific endopeptidase which generates angiotensin I from angiotensinogen in the plasma. angiotensin I is an important regulator of blood pressure and electrolyte balance. Renin initiates a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Description
REN produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 387 amino acids (22-402 a.a.) and
having a molecular mass of 42.5kDa (Migrates at 40-57kDa on SDS-PAGE under
reducing conditions).
REN is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
REN protein solution (0.25mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
LPTRTATFER IPLKKMPSVR EILEERGVDM TRLSAEWGVF TKRPSLTNLT
SPVVLTNYLN TQYYGEIGIG TPPQTFKVIF DTGSANLWVP STKCSRLYLA CGIHSLYESS DSSSYMENGS
DFTIHYGSGR VKGFLSQDSV TVGGITVTQT FGEVTELPLI PFMLAKFDGV LGMGFPAQAV GGVTPVFDHI
LSQGVLKEEV FSVYYNRGSH LLGGEVVLGG SDPQHYQGNF HYVSISKTDS WQITMKGVSV GSSTLLCEEG
CAVVVDTGSS FISAPTSSLK LIMQALGAKE KRIEEYVVNC SQVPTLPDIS FDLGGRAYTL SSTDYVLQYP
NRRDKLCTLA LHAMDIPPPT GPVWVLGATF IRKFYTEFDR HNNRIGFALA RHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
