Catalogue number
PRO-1911
Synonyms
CD40 Molecule, TNF Receptor Superfamily Member 5, TNFRSF5,
Tumor Necrosis Factor Receptor Superfamily, Member 5, Bp50, B-Cell Surface
Antigen CD40, CD40L Receptor, CDW40, B Cell Surface Antigen CD40, B
Cell-Associated Molecule, CD40 Antigen (TNF Receptor Superfamily Member 5), CD40
Type II Isoform, Nerve Growth Factor Receptor-Related B-Lymphocyte Activation
Molecule, p50, Tumor Necrosis Factor Receptor Superfamily Member 5, CDw40, CD40
Antigen.
Introduction
CD40
belongs to the TNF-receptor super family. CD40 has been found to be vital in mediating
a wide range of immune and inflammatory responses including T cell-dependent
immunoglobulin class switching,
memory B cell development, and germinal center
formation. AT-hook transcription factor AKNA is accounted to coordinately regulate the
expression of CD40 and its ligand, which is significant for homotypic cell interactions. Adaptor
protein TNFR2 interacts with CD40 and functions as a mediator of the signal transduction.
The interaction of CD40 and its ligand is found to be essential for
amyloid-beta-induced microglial
activation, and therefore is considered to be an early event in Alzheimer
disease pathogenesis.
Description
CD40 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 196 amino acids (21-193a.a) and
having a molecular mass of 21.6kDa.
CD40 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CD40 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSEPPTACR EKQYLINSQC CSLCQPGQKL VSDCTEFTET ECLPCGESEF
LDTWNRETHC HQHKYCDPNL GLRVQQKGTS ETDTICTCEE GWHCTSEACE SCVLHRSCSP GFGVKQIATG
VSDTICEPCP VGFFSNVSSA FEKCHPWTSC ETKDLVVQQA GTNKTDVVCG PQDRLR
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
