Catalogue number
CYT-316
Synonyms
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4.
Introduction
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E- cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.
Description
CD-4 Human Recombinant His Tag protein encoding 203-317 amino acids.
Source
Escherichia Coli.
Physical Appearance
Sterile lyophilized powder.
Formulation
Each mg contains 1X PBS, 0.1% SDS and 1mM DTT.
Stability
Store vial at -20oC to -80oC. When stored at the recommended temperature, this protein is stable for 12 months.
Please avoid freeze-thaw cycles.
Applications
• Protein Assay.
• Antibody Production.
• Western Blotting.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Safety Data Sheet
Solubility
It is recommended to reconstitute the lyophilized CD-4 (203-317)in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
