Catalogue number
PRO-1459
Synonyms
ALS10,
TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.
Introduction
TAR
DNA Binding Protein (TARDBP) was initially recognized as a transcriptional repressor
which binds to chromosomally integrated TAR DNA and represses HIV-1
transcription. In addition, TARDBP has been detected in individuals diagnosed
with chronic traumatic encephalopathy as well, which is a condition that often
imitates ALS and has
been related with athletes who have experienced multiple concussions and other
types of head injury.TARDBP
has been shown to bind both DNA and RNA, it has multiple functions in
transcriptional repression, pre-mRNA splicing and translational regulation.
Description
TARDBP Human Recombinant produced in E.Coli is a single,
non-glycosylated, polypeptide chain containing 450 amino acids (1-414 a.a) and
having a total molecular mass of 48.8 kDa. TARDBP is fused to a 36 amino acid His-tag at
N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
TARDBP
protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol
and 0.4M urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNAEPKH NSNRQLERSG RFGGNPGGFG NQGGFGNSRG GGAGLGNNQG SNMGGGMNFG AFSINPAMMA AAQAALQSSW GMMGMLASQQ NQSGPSGNNQ NQGNMQREPN QAFGSGNNSY SGSNSGAAIG WGSASNAGSG SGFNGGFGSS MDSKSSGWGM.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
