Catalogue number
PRO-1464
Synonyms
Vesicle transport protein USE1, Putative MAPK-activating
protein PM26, USE1-like protein, p31, USE1L, MDS032, Q-SNARE, SLT1, SNARE-Like
Tail-Anchored Protein 1 Homolog, Protein P31.
Introduction
USE1
Belongs to the USE1 family, this protein is a vesicle transport protein. USE1
is a component of a SNARE
complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B. In addition
USE1 interacts directly with STX18. SNARE
may be involved
in targeting and fusion of Golgi-derived retrograde transport vesicles with the
ER. Diseases associated with USE1 comprise dysentery and hemolytic-uremic
syndrome. Among its related super-pathways are Nicotine Pathway (Dopaminergic
Neuron) and Pharmacodynamics. GO annotations related to this gene include
protein binding.
Description
USE1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 254 amino acids (1-231 a.a) and having a molecular mass of 28.3kDa.
USE1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
USE1 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAASRLE LNLVRLLSRC EAMAAEKRDP DEWRLEKYVG ALEDMLQALK
VHASKPASEV INEYSWKVDF LKGMLQAEKL TSSSEKALAN QFLAPGRVPT TARERVPATK TVHLQSRARY
TSEMRSELLG TDSAEPEMDV RKRTGVAGSQ PVSEKQSAAE LDLVLQRHQN LQEKLAEEML GLARSLKTNT LAAQSVIKKD NQTLSHSLKM
ADQNLEKLKT ESERLEQHTQ KSVN
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
