Catalogue number
CYT-863
Synonyms
Interleukin-34, IL-34, Il34.
Introduction
Interleukin 34 (IL34) is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor and is a part of a group of cytokines called interleukins. IL34 increases growth or survival of immune cells known as monocytes. IL34 protein obtains its activity by binding the Colony stimulating factor 1 receptor. Interleukin 34 has also a potential part in viral infection, the adaptive immune response and bone marrow cell proliferation. Furthermore, IL34 plays an important role in innate immunity and in inflammatory processes.
Description
IL34 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (21-235 a.a) and having a molecular mass of 26.8kDa.
IL34 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
IL34 protein solution (0.5mg/ml) in 20mM Tris-HCl buffer (pH 8.0) and 10% Glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MNENLEIWTL TQDKECDLTG YLRGKLQYKN RLQYMKHYFP INYRIAVPYE GVLRVANITR
LQKAHVSERE LRYLWVLVSL NATESVMDVL LEGHPSWKYL QEVQTLLENV QRSLMDVEIG PHVEAVLSLL
STPGLSLKLV RPKALLDNCF RVMELLYCSC CKQSPILKWQ DCELPRLHPH SPGSLMQCTA TNVYPLSRQT
PTSLPGSPSS SHGSLP.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
