Catalogue number
PRO-816
Synonyms
Hqk, hqkI, QK, QK1, QK3, Protein quaking, Quaking
Homolog, KH Domain RNA Binding, RNA Binding Protein HQK, HKQ, KH
Domain Containing, RNA Binding.
Introduction
KH Domain RNA Binding also known as QKI is a
RNA-binding protein which regulates pre-mRNA splicing, export of mRNAs from the
nucleus, protein translation, and mRNA stability. QKI
is involved in myelinization
and oligodendrocyte differentiation, and it may play a role in schizophrenia.
Defects or deletions in the QKI
are linked with astrocytic tumors and may be implicated in the pathogenesis of
schizophrenia. Among the diseases associated with QKI are
6q terminal deletion syndrome, and schizophrenia.
Description
QKI Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 364 amino acids (1-341 a.a) and having a molecular mass of 40.1kDa.
QKI is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
QKI protein solution (0.25mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMVGEMET KEKPKPTPDY LMQLMNDKKL MSSLPNFCGI FNHLERLLDE EISRVRKDMY NDTLNGSTEK RSAELPDAVG PIVQLQEKLY VPVKEYPDFN FVGRILGPRG LTAKQLEAET GCKIMVRGKG SMRDKKKEEQ NRGKPNWEHL NEDLHVLITV EDAQNRAEIK LKRAVEEVKK LLVPAAEGED SLKKMQLMEL AILNGTYRDA NIKSPALAFS LAATAQAAPR IITGPAPVLP PAALRTPTPA GPTIMPLIRQ IQTAVMPNGT PHPTAAIVPP GPEAGLIYTP YEYPYTLAPA TSILEYPIEP SGVLGAVATK VRRHDMRVHP YQRIVTADRA ATGN.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
