Catalogue number
HSP-060
Synonyms
DnaJ homolog subfamily C member 27, DnaJ (Hsp40) Homolog, Subfamily C, Member 27, DNAJC27, Rab and DnaJ domain-containing protein, RABJS, RBJ, dnaJ homolog subfamily C member 27 isoform 1.
Introduction
DnaJ (Hsp40) Homolog, Subfamily C, Member 27 (DNAJC27) which contains one J domain is a part of the small GTPase superfamily. DNAJC27 is a GTPase which activates the MEK/ERK pathway and induces cell transformation when overexpressed.
Description
DNAJC27 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 296 amino acids (1-273 a.a.) and having a molecular mass of 33.2kDa.
DNAJC27 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The DNAJC27 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 50% glycerol, 2mM DTT and 2mM EDTA.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMEANMPK RKEPGRSLRI KVISMGNAEV GKSCIIKRYC EKRFVSKYLA TIGIDYGVTK VHVRDREIKV NIFDMAGHPF FYEVRNEFYK DTQGVILVYD VGQKDSFDAL DAWLAEMKQE LGPHGNMENI IFVVCANKID CTKHRCVDES EGRLWAESKG FLYFETSAQT GEGINEMFQT FYISIVDLCE NGGKRPTTNS SASFTKEQAD AIRRIRNSKD SWDMLGVKPG ASRDEVNKAY RKLAVLLHPD KCVAPGSEDA FKAVVNARTA LLKNIK.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
