Catalogue number
PRO-2213
Synonyms
Complement Factor D (Adipsin), Properdin Factor D, D
Component Of Complement (Adipsin), C3 Convertase Activator, EC 3.4.21.46,
ADIPSIN, PFD, AND, DF, Complement Factor D Preproprotein, Complement Factor D,
EC 3.4.21, Complement factor D.
Introduction
Complement Factor D (Adipsin), which belongs to the trypsin family of peptidases, is involved in the alternative complement pathway of the complement system where it cleaves factor B. In the alternative complement pathway, Adipsin is best known for its role in humoral suppression of infectious agents. In addition, Adipsin is a serine protease which is secreted by adipocytes into the bloodstream. Ultimately, Adipsin has a high level of expression in fat, proposing a role for adipose tissue in immune system biology.
Description
Adipsin produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 241 amino acids (21-253a.a.) and
having a molecular mass of 26.01kDa. (Molecular size on SDS-PAGE will appear at
approximately 28-40kDa).
Adipsin is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
Adipsin
protein solution (1mg/ml) contains phosphate buffered saline (pH7.4).
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
PPRGRILGGR EAEAHARPYM
ASVQLNGAHL CGGVLVAEQW VLSAAHCLED AADGKVQVLL GAHSLSQPEP SKRLYDVLRA VPHPDSQPDT IDHDLLLLQL
SEKATLGPAV RPLPWQRVDR DVAPGTLCDV AGWGIVNHAG RRPDSLQHVL LPVLDRATCN RRTHHDGAIT
ERLMCAESNRRDSCKGDSGG PLVCGGVLEG
VVTSGSRVCG NRKKPGIYTR VASYAAWIDS VLAVEHHHHH H.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
