Catalogue number
PRO-127
Synonyms
RPP1, RRP1, 60S acidic ribosomal protein P1, FLJ27448, MGC5215, acidic ribosomal phosphoprotein P1.
Introduction
Ribosomes, the organelles which catalyze protein synthesis, contain a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and nearly 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein which is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, is a member of the L12P family of ribosomal proteins and has a vital part in the elongation step of protein synthesis. In opposed to most ribosomal proteins, which are basic, the encoded protein is acidic.The P1 C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. P1 is located in the cytoplasm. Two alternatively spliced transcript variants which encode varies proteins have been detected. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene distributed all over the genome.
Description
RPLP1 is a full-length cDNA coding for the human ribosomal P1 phosphoprotein having a molecular mass of 12,336 Dalton (pH 4.75). RPLP1 protein is fused to a hexa-histidine purification tag.
Source
Sf9 insect cells.
Formulation
RPLP1 (0.34mg/ml) is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 6M Urea.
Immunological functions
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checkerboard analysis of positive/negative sera panels).
Applications
Western blot with SLE sera (Systemic Lupus Erythematosus) ormonoclonal antihexa-His-tag antibody.
Coating concentration
0.3-0.7 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
