Catalogue number
PRO-1465
Synonyms
L23A, MDA20, 60S ribosomal protein L23a, RPL23A, Melanoma
Differentiation-Associated Gene 20.
Introduction
RPL23A is a ribosomal protein which is a component of the 60S subunit. Ribosomes, the organelles which catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally different proteins. RPL23A is a member of the L23P family of ribosomal proteins. It is located in the cytoplasm. Recombinant Human Ribosomal Protein L23A may be one of the target molecules involved in mediating growth inhibition. The corresponding protein binds to a specific site on the 26S rRNA in yeast.
Description
RPL23A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids (1-156 a.a) and having a molecular mass of 20.1kDa.
RPL23A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
RPL23A protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT and 250mM imidazole.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAPKAKK EAPAPPKAEA KAKALKAKKA VLKGVHSHKK KKIRTSPTFR
RPKTLRLRRQ PKYPRKSAPR RNKLDHYAII KFPLTTESAM KKIEDNNTLV FIVDVKANKH QIKQAVKKLY
DIDVAKVNTL IRPDGEKKAY VRLAPDYDAL DVANKIGII
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
