Catalogue number
PRO-2319
Synonyms
Complement decay-accelerating factor, GPI-anchored,
DAF-GPI, CD_antigen: CD55.
Introduction
CD55
belongs to the RCA (regulators of complement activation) family characterized
by 4-30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 is
widely expressed in malignant tumors and nevertheless functions as an inhibitor
of the complement system.
Description
CD55 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 336 amino acids (35-362 a.a.) and
having a molecular mass of 36.8kDa (Migrates at 40-57kDa on SDS-PAGE under
reducing conditions).
CD55 is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
CD55
protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10%
glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
DCGPPPDIPN ARPILGRHSK FAEQSKVAYS CNNGFKQVPD KSNIVVCLEN
GQWSSHETFC EKSCVAPERL SFASLKKEYL NMNFFPVGTI VEYECRPGFR KQPPLPGKAT CLEDLVWSPV
AQFCKKKSCP NPKDLDNGHI NIPTGILFGS EINFSCNPGY RLVGVSSTFC SVTGNTVDWD DEFPVCTEIH
CPEPPKINNG IMRGESDSYT YSQVVTYSCD KGFILVGNAS IYCTVSKSDV GQWSSPPPRC IEKSKVPTKK
PTINVPSTGT PSTPQKPTTE SVPNPGDQPT PQKPSTVKVS ATQHVPVTKT TVRHPIRTST DKGEPNTGLE
HHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
