Catalogue number
PRO-1756
Synonyms
Dynein, Axonemal, Light Intermediate Chain 1, DJ423B22.5
(Axonemal Dynein Light Chain (Hp28)), Inner Dynein Arm Light Chain Axonemal,
hp28, P28, Homolog Of Clamydomonas, Axonemal Dynein Light Intermediate
Polypeptide 1, dJ423B22.5, Inner Dynein Arm,
Homolog Of Clamydomonas, Dynein, Axonemal, Light Intermediate Polypeptide 1.
Introduction
Dynein
Axonemal Light Intermediate Chain 1 also known as DNALI1 belongs to the
inner dynein arm light chain family. DNALI1 take a dynamic role in flagellar
motility. DNALI1 is the human homolog of the Chlamydomonas inner dynein arm
gene, p28. The exact function of DNALI1 is not known
yet, on the other hand, it is a potential candidate for immotile cilia
syndrome. In addition it has been found that the following diseases: primary
ciliary dyskinesia, and huntington's disease have been associated with DNALI1. Ultrastructural
defects of the inner dynein arms are seen in patients with ICS. Immotile mutant
strains of Chlamydomonas, biflagellated algae,
show similar defects.
Description
DNALI1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (1-280 a.a) and having a molecular mass of 34.2kDa. DNALI1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
DNALI1 protein solution (1mg/ml)
containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol, 1mM DTT and
0.1mM PMSF.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMVTANKA HTGQGSCWVA TLASAMIPPA DSLLKYDTPV LVSRNTEKRS
PKARLLKVSP QQPGPSGSAP QPPKTKLPST PCVPDPTKQA EEILNAILPP REWVEDTQLW IQQVSSTPST
RMDVVHLQEQ LDLKLQQRQA RETGICPVRR ELYSQCFDEL IREVTINCAE RGLLLLRVRD
EIRMTIAAYQ TLYESSVAFG MRKALQAEQG KSDMERKIAE LETEKRDLER QVNEQKAKCE ATEKRESERR
QVEEKKHNEE IQFLKRTNQQ LKAQLEGIIA PKK
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
