Catalogue number
PRO-1856
Synonyms
GIF, GIFB, GRIF, ZnMT3, Metallothionein-3, MT-3, Growth inhibitory factor, Metallothionein-III, MT-III, MT3.
Introduction
Metallothionein 3 (MT3) contains 3 zinc and 3 copper atoms per polypeptide chain and a minor amount of cadmium. MT3 inhibits survival and neurite formation of cortical neurons in vitro. MT3 also binds heavy metals.
Description
MT3 Human Recombinant produced in E. coli is a single polypeptide chain containing 91 amino acids (1-68) and having a molecular mass of 9.3kDa (molecular size on SDS-PAGE will appear higher).
MT3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The MT3 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMDPETCP CPSGGSCTCA DSCKCEGCKC TSCKKSCCSC CPAECEKCAK DCVCKGGEAA EAEAEKCSCC Q.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
