Catalogue number
PRO-1124
Synonyms
Myosin light chain 12B regulatory, Myosin regulatory light chain 2-B smooth muscle isoform, Myosin regulatory light chain 20 kDa, Myosin regulatory light chain MRLC2, MYLC2B, MRLC2, MLC-2A, MLC-B, SHUJUN-1 MLC20.
Introduction
Myosin is composed of 2 nonphosphorylatable alkali light chains, 2 heavy chains and 2 phosphorylatable regulatory light chains. MYL12B is a hexameric ATPase cellular motor protein which controls contraction in smooth muscle and non-muscle cells through phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains catalyzed by MLCK in the presence of calcium and calmodulin, rises Actin-activated myosin ATPase activity, thus regulating contractile activity.
Description
MYL12B Human Recombinant produced in E. coli is a single polypeptide chain containing 196 amino acids (1-172) and having a molecular mass of 22.3kDa.
MYL12B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The MYL12B solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM Nacl, 2mM DTT and 40% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
