Catalogue number
PRO-1888
Synonyms
DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, MHC class II antigen
DRB1 16, DR-16, DR16, Human Leucocyte AntigenDRB1, MHC Class
IIHLA-DR-Beta Cell Surface Glycoprotein, MHC Class IIHLA-DRw10-Beta.
Introduction
Major Histocompatibility Complex Class II DR Beta 1 also known as HLA-DRB1 ia a member of the HLA class II beta chain paralogs. Molecule class II is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. HLA-DRB1 takes an essential part in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). Furthermore, the beta chain is approximately 26- 28 kDa. It is encoded by 6 exons. While exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail.
Description
HLA-DRB1 Human Recombinant produced in E.Coli is a
single, non-glycosylated polypeptide chain containing 219 amino acids (30-227 a.a)
and having a molecular mass of 25.2kDa.
HLA-DRB1 is fused to a 21 amino acid His-tag at
N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
HLA-DRB1 protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGDTRPRFLW QPKRECHFFN GTERVRFLDR YFYNQEESVR FDSDVGEFRA VTELGRPDAE
YWNSQKDILE QARAAVDTYC RHNYGVVESF TVQRRVQPKV TVYPSKTQPL QHHNLLVCSV SGFYPGSIEV
RWFLNGQEEK AGMVSTGLIQ NGDWTFQTLV MLETVPRSGE VYTCQVEHPS VTSPLTVEWR ARSESAQSK
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
